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Methodsofscreening,culturing,improvementandstorageofmicroorganisms...
5.
Apparatus:gaschromatographAgilentGC7890withmass
spectrometerMS5975C.
Aimoftheexercise
Obtainingstrainsofmicroorganismscapableofbiosynthesisofbiologicallyactve
substancesanddecompositonofpetroleumderivatves.
Procedure
1.Prepareasoilextract.
Weigh2gofsoil,transfertoa100mlflaskcontaining
20mlof0.85%NaClsolutionandaddsterileglassbeads.
Incubateonashakerfor0.5hatroomtemperature.Once
theincubationiscomplete,wait2-3minuntilthelarger
soilparticleshavesettled.
2.
Makeaseriesofdilutionsofthesoilextractin0.85%NaCl
solutionaccordingtothefollowingscheme:
Fig0102050Seriesof
dilufionsofthesoil
extract
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