Treść książki
Przejdź do opcji czytnikaPrzejdź do nawigacjiPrzejdź do informacjiPrzejdź do stopki
ĆWICZENIE5.HybrydyzacjaRNAnamembraniezsondąradioaktywną
specyficznąwobecIGS1-R,wyznakowanąmetodą„random-priming”lllllllllllllllll
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3lWykrywaniemałychRNAlllllllllllllllllllllllllllllllllllllllll25
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MałeinterferująceRNA:miRNAisiRNAlllllllllllllllllllllllllllllllllllllllllllllllllllll
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WybranemetodydetekcjimałychRNA(iRNA)llllllllllllllllllllllllllllllllllllllllllllllll
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Literaturalllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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ĆWICZENIE4.PulsacyjnyRT-PCR,cz.1–pulsacyjnaodwrotna
transkrypcjallllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
Materiałylllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
TrawienieDNaząlllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
Pulsacyjnaodwrotnatranskrypcjalllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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ĆWICZENIE5.ReakcjaPCR–cz.2llllllllllllllllllllllllllllllllllllllllllllllll
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ĆWICZENIE6.RozdziałwżeluagarozowymproduktówpulsacyjnegoRT-PCRlllllllllll
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Przygotowaniepróbekllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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4lBadaniekońcówcząsteczekRNAnaprzykładziedrożdżowych
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RNazaHllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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LigacjaRNAlllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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Literaturalllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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ĆWICZENIE6.TrawienieRNaząHiligacjaRNAllllllllllllllllllllllllllllllllllll
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TrawienieRNaząHllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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LigacjaRNAlllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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ĆWICZENIE7.ReakcjaodwrotnejtranskrypcjiiPCRllllllllllllllllllllllllllllllll
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Reakcjaodwrotnejtranskrypcjillllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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PCR
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ĆWICZENIE8lElektroforezaproduktówPCRlllllllllllllllllllllllllllllllllllll
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5lPCRilościowy:RT-qPCRllllllllllllllllllllllllllllllllllllllllll39
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FormatydetekcjiproduktówqPCRlllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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KontrolajakościRNAllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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AnalizadoświadczeńqPCRlllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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NajbardziejkluczoweelementyRT-qPCRllllllllllllllllllllllllllllllllllllllllllllllllllll
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Literaturalllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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ĆWICZENIE8.ProjektowaniestarterówdoqPCR–konkursnanajlepsząparęstarterówll
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Wskazówkidoprojektowaniastarterówllllllllllllllllllllllllllllllllllllllllllllllllllll
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Wykonaniećwiczenialllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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ĆWICZENIE9.TestowaniezaprojektowanychstarterówdoqPCRlllllllllllllllllllll
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Przygotowaniestarterówllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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PrzygotowaniereakcjiqPCRllllllllllllllllllllllllllllllllllllllllllllllllllllllllllllll
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ĆWICZENIE10.AnalizareakcjiqPCRllllllllllllllllllllllllllllllllllllllllllll
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