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Spistreści/Contents
9.3.Określanieaktywnościlipazwefrakcjachmikrosomalnych
zróżnegomateriałuroślinnego.......................................
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79
Laboratoryworkshopguide
forbiochemicalandbiophysicalbasis
ofplantdevelopment
Forewordnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn85
1.Growthanddevelopmentofplantsnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn87
1.1.Theeffectofdayandnightlengthonplantdevelopment(photoperiodism)
87
1.2.Theimpactofacoldperiodonplantdevelopment
(thephenomenonofvernalization)...................................
88
1.3.Theeffectoflightonplantgrowth....................................
89
1.4.Seedstructureandtypesofgermination...............................
89
1.5.Germinationenergyandgerminationcapacity..........................
90
1.6.Biochemicalmethodsofassessingseedviability.........................
92
1.7.Breakingthedormancyofpotatotubers...............................
93
2.Plant–waterrelationsInnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn95
2.1.Swellingoflivinganddeadseeds.....................................
95
2.2.Theeffectofdifferentionsonseeds’rateofswelling.....................
96
2.3.Heatemissionduringswelling........................................
97
2.4.Traubecell..........................................................
98
2.5.Plasmolysisanddeplasmolysis........................................
99
2.6.Measuringtheosmoticpotentialofapotatotuberwithanalyticalbalance..100
2.7.Measuringtheosmoticpotentialinonioncells
usingtheincipientplasmolysismethod................................101
3.Plant–waterrelationsIInnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn103
3.1.Demonstrationoftranspirationpull
(passivemechanismofwaterabsorption)..............................103
3.2.Demonstrationoftheactivemechanismofwaterabsorption.............104
3.3.Microscopicobservationofleafepidermiscells
thestructureandmovementsofstomata..............................104
3.4.Microscopicobservationofwater-conductingtissues
ofpeaepicotylandthepedicelofaselectedwhiteflower................105
3.5.Therateofstomatalandcuticulartranspiration.........................106
3.6.Theroleofcoveringtissuesinpreventingwaterloss.....................107
3.7.Theweightmethodoftranspirationmeasurement......................108
3.8.Samplepreparationandobservationofcellwallsunderthemicroscope...110
4.Photosynthesisnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn112
4.1.Extractionofphotosyntheticpigments.................................112
4.2.Photosyntheticpigmentseparation....................................113