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Spistreści/Contents
4.3.Chemicalpropertiesofphotosyntheticpigments........................115
4.4.Physicalpropertiesofphotosyntheticpigments.........................117
4.5.Measuringchlorophyllandcarotenoidcontent
withtheabsorptionspectrophotometrymethod........................118
4.6.Productsofphotosynthesisingeraniumandonionleaves................119
4.7.Thenecessityofcarbondioxideintheprocessofphotosynthesis..........121
4.8.TheactivityofNADPH-dependentmalatedehydrogenase
inC
4andC
3plants..................................................122
4.9.Samplepreparationandobservation
ofthechloroplaststructureunderthemicroscope......................124
5.Respirationnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn126
5.1.Determinationoftherespiratoryquotientofgerminatingseeds...........126
5.2.Effectofthetemperatureontheintensityofrespiration..................127
5.3.Intensityofrespirationofdry,swollenandgerminatingseeds............129
5.4.Storagereservesmobilizationinthegerminatingseeds..................130
6.Mineralnutritionnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn133
6.1.Elementspresentinplants–ashanalysis...............................133
6.2.Detectionofammoniumionsinthefreshplantmaterial.................135
6.3.Detectionofnitratesinthefreshplantmaterial.........................136
6.4.Activityofnitratereductaseintheleavesofnitrate-fertilized
andnitrate-unfertilizedseedlings.....................................137
7.Plantlipidsnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn139
7.1.Lipidextractionfromdifferentplantmaterials..........................139
7.2.Determinationofthetotallipidcontentinchloroformextracts.............140
7.3.Analysisofthecontentofindividuallipidclasses
inthetestedplantmaterial...........................................141
8.Isolationofcellorganellesnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn145
8.1.Isolationofmicrosomalfractionsfromtheplantmaterial.................145
8.2.Isolationofchloroplasts..............................................146
9.Enzymesoflipidmetabolismnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn149
9.1.Determinationoftheactivityofacyl-CoA:diacylglycerol
acyltransferase(DGAT)inmicrosomalfractionsoftestedplantmaterial...149
9.2.Determinationoftheactivityofacyl-CoA:lysophosphatidylcholine
acyltransferase(LPCAT)inmicrosomalfractions
isolatedfromtestedplantmaterial....................................151
9.3.Determinationoftheactivityoflipasesinmicrosomalfractions
oftestedplantmaterial..............................................153
Literatura/Literaturennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn156
