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Microbialbiotechnologyinthelaboratoryandinpractce
Thescreeningstageincludestheselectionofthecollection
siteandthemethodofisolatingmicroorganismsusing
appropriatemedia.Ifwearelookingforprotease-producing
bacteria,aconvenientplacetocollectsamplesmaybe,among
others,theareasaroundindustrialplantsproducingsignificant
amountsofwastecontainingproteins,e.g.dairies,meatplants.
Atafurtherstage,puremicrobialculturesareisolated,on
thebasisofwhichliquidculturesareprepared.Apreliminary
assessmentofthesuitabilityoftheisolatedstrainsforusein
aspecifictechnologicalprocessismade.Ifaspecificproductis
desired,processoptimisationiscarriedout,oftencombinedwith
improvingtheisolatedstrainandincreasingthescaleofculture.
Thefinalstageofscreeningincludespurificationoftheproduct
(ifnecessary),patentingoftherelevant(key)productionsteps
andcommercialisationoftheobtainedresearchresults.Thekey
stagesofscreeningareshowninFigure1.2.1.
Fig0102010Stages
ofscreening
microorganisms
thatareusefulin
biotechnological
processes.
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