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2.SAFETYiNTHECLiNiCAL
ORMiCROBiOLOGYLABORATORY
Anyclinicalormicrobiologylaboratoryisapotentiallyhazardous
place.However,itistheattitudeofthepersonnelthatmakesa
laboratoryasafeorunsafeplacetowork.Ithasbeenestablished
thatignoranceandcarelessnessarethetwoofmostcommoncauses
oflaboratoryaccidents.Studiesoflaboratory-associatedinfections
haveindicatedthatmostsuchinfectionsdonotresultfromknown
exposuresandaccidentsbutinsteadfromroutinehandlingofbiologic
orpatientspecimens.
Tefollowingrulestogetherwiththegeneralruleslistedinsection
KGeneralRequirementsforPersonalSafetyinaLaboratoryArea”
shouldbethenormforallclinicalormicrobiologylaboratories:
l
Laboratoryandsupportpersonnelmusthaveknowledgeofthe
biologicalrisksintheclinicalormicrobiologicallaboratoryandact
accordingly.
l
BecauseofthespecialrisksassociatedwithAIDS,allclinical
specimensshouldbetreatedasiftheycontainHIV.Latexor
vinylglovesshouldbewornwheneverhandlingspecimensofany
kind.
l
Masksmustbewornanytimethereisapossibilityofgenerating
anaerosolduringspecimenpreparation.
l
Electiveproceduresfordecontaminatinginfectiousmaterialsor
wastes(i.e.specimens,syringesandneedles,inoculatedmedia,
bacterialcultures,tissuecultures,experimentalanimals,glassware,
instruments,andsurfaces)mustbeinplaceandpracticedwithout
compromise.
l
A5,25%(fullstrength)chlorinebleachsolutionisrecommended
fordecontaminatingspilledinfectiousmaterial.
l
Allpotentiallyinfectiouswastemustbeburnedinacertified
incineratororhandledbyalicensedwastehandler.
l
Personnelworkingwithhazardousinfectiousagentsorvaccines
(e.g.rabies,polio,ordiphtheria-pertussis-tetanusvaccines)must
bevaccinatedagainsttheagent.
l
Personnelworkingwithhumanorprimatetissuemustbe
vaccinatedagainstHBV
.
l
Allpipettingmustbedonewithautomaticpipettingdevices(not
bymouth).
l
Laboratorypersonnelmustwearlaboratorycoats(andloraprons),
sealedshoes,rubbergloves,masks,eyeprotection,respiratory
deviceswhenneeded,andotherbarrierprotectionasdeemed
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